![]() Similarly, Sudanese sera taken soon after treatment did not show a significant change in the IgG1 levels. donovani antigen-specific IgG1 immunochromatographic rapid diagnostic tests (RDTs) were developed and consisted of a cassette with a nitrocellulose membrane, a sample pad, a conjugate pad and an absorbent pad, backed with a plastic strip.įor the Indian VL sera, consistent with the known IgG1 half-life, IgG1 levels had not decreased significantly at day 30 after the start of treatment, but were dramatically decreased by six months compared to day 0 or day 15 after the start of treatment. The ELISAs were read at 490 nm on a Spectra Max 190 microplate reader (Molecular Devices Sunnyvale, CA, USA), the MRX II plate reader, (Dynex Technologies Chantilly, USA). Leishmania donovani specific enzyme-linked immunosorbent assays (ELISA) were performed and human immunoglobulin G (IgG) isotype responses were determined. Serum samples were also collected in 20, from active VL, treated, relapsed, PKDL, and endemic controls in eastern Sudan. ![]() ![]() ![]() Scientists at the London School of Hygiene and Tropical Medicine (UK) and their international colleagues collected plasma samples after 2007 from active VL, cured, relapsed, post-kala-azar dermal leishmaniasis (PKDL) and asymptomatic groups from the endemic region of Bihar state (north-eastern India), and control subjects from a region where VL is not endemic. The most sensitive and specific method to detect the causative agent of VL is microscopic examination of invasive spleen aspirates bone marrow and lymph node aspirates provide similar high specificity but lesser sensitivity although more user-friendly point-of-care (POC) diagnostics have been developed based on antibody detection. ![]() The effective clinical management, chemotherapy and control of the transmission of visceral leishmaniasis (VL) are largely dependent on early and unequivocal diagnosis as without effective chemotherapy symptomatic VL is usually fatal. ![]()
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